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	Provedor de dados ArchiMer (2) Autor Le Bris, Cedric (2) Paillard, Christine (2) Bidault, Adeline (1) Gauthier, Olivier (1) Guerard, Fabienne (1) Lambert, Christophe (1) Pernet, Fabrice (1) Richard, Gaelle (1) Richard, Gaelle G. (1) Seguineau, Catherine (1) Mais... Palavra-chave Venerupis philippinarum (2) Vibrio tapetis (2) Brown ring disease (1) Immunity (1) Marine pathogen (1) Molecular diagnostic (1) Phenoloxidase (1) Superoxide dismutase (1) Taqman real-time PCR (1) VirB4 gene (1) Mais... Tipo do documento Text (2) Ano 2015 (2) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of a Taqman real-time PCR assay for rapid detection and quantification of Vibrio tapetis in extrapallial fluids of clams ArchiMer Bidault, Adeline; Richard, Gaelle G.; Le Bris, Cedric; Paillard, Christine. The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600(T) V. tapetis strain. Quantification curves of... Tipo: Text Palavras-chave: Vibrio tapetis; VirB4 gene; Taqman real-time PCR; Molecular diagnostic; Venerupis philippinarum; Marine pathogen; Brown ring disease. Ano: 2015 URL: https://archimer.ifremer.fr/doc/00308/41909/73194.pdf Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection ArchiMer Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne. Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection.... Tipo: Text Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase. Ano: 2015 URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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